Enter a DNA sequence, or select from other options, to identify cut sites. Once you submit a sequence, you may choose to customize your digest.
1. Input or choose sequence.
2. Set preferences.
3. Name project (optional).

Select one of the following methods to enter your desired sequence:

  • “Paste Sequence”: cut and paste your sequence into the box. Either plain DNA sequence or a FASTA formatted sequence is acceptable. Alternatively, a short sequence can be typed in directly.

  • “Upload File”: Click “Browse” and select the desired file from your computer.

  • “Use GenBank”: Enter a valid Genbank number (If you do not know the GenBank number of your sequence, Click the GenBank link).

  • “Select Plasmid Vector”: Select from a set of pre-populated plasmid vectors by using the pulldown menu. Alternatively, start typing in the name of your plasmid and it will come up if available.

  • “Select Viral & Phage”: Select from a set of pre-populated viral and phage sequences by using the pulldown menu. Alternatively, start typing in the name and it will come up if available.

  • Check “Circular” if your sequence is circular. If not, please leave blank.

  • Click “More” to see further options, including: to add other enzyme Types (I and/or III, homing and nicking), to specify one or more methylation sensitivities, to specify minimum ORF length, to change the genetic code to be used, or to specify a sub-region within the input file. You can also define up to 40 oligonucleotide sequences of your choice.